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sample purification beads  (New England Biolabs)


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    New England Biolabs sample purification beads
    Expression and <t>purification</t> of recombinant His-MBP-FOXP3(ΔN) protein (A) Schematic flowchart of the protein purification process, including Ni-NTA affinity chromatography followed by HiTrap Heparin HP chromatography. (B) SDS-PAGE analysis of purified fractions during the HiTrap Heparin HP chromatography. The arrow indicates the target protein. M represents the molecular weight marker, with numbers on the right indicating molecular weights in kDa. The gel was stained with Coomassie Blue dye. (C) The HiTrap Heparin HP affinity chromatography profile of the final purified protein. An asterisk indicates the peak corresponding to the desired FOXP3.
    Sample Purification Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1067 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e7103s/NEBNext+Ultra+II+DNA+Library+Prep+with+Sample+Purification+Beads/pmc13123352-224-12-15
    Average 99 stars, based on 1067 article reviews
    sample purification beads - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Protocol for the genome-wide identification of intrinsic transcription factor binding motifs by mammalian-optimized pull-down sequencing"

    Article Title: Protocol for the genome-wide identification of intrinsic transcription factor binding motifs by mammalian-optimized pull-down sequencing

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2026.104513

    Expression and purification of recombinant His-MBP-FOXP3(ΔN) protein (A) Schematic flowchart of the protein purification process, including Ni-NTA affinity chromatography followed by HiTrap Heparin HP chromatography. (B) SDS-PAGE analysis of purified fractions during the HiTrap Heparin HP chromatography. The arrow indicates the target protein. M represents the molecular weight marker, with numbers on the right indicating molecular weights in kDa. The gel was stained with Coomassie Blue dye. (C) The HiTrap Heparin HP affinity chromatography profile of the final purified protein. An asterisk indicates the peak corresponding to the desired FOXP3.
    Figure Legend Snippet: Expression and purification of recombinant His-MBP-FOXP3(ΔN) protein (A) Schematic flowchart of the protein purification process, including Ni-NTA affinity chromatography followed by HiTrap Heparin HP chromatography. (B) SDS-PAGE analysis of purified fractions during the HiTrap Heparin HP chromatography. The arrow indicates the target protein. M represents the molecular weight marker, with numbers on the right indicating molecular weights in kDa. The gel was stained with Coomassie Blue dye. (C) The HiTrap Heparin HP affinity chromatography profile of the final purified protein. An asterisk indicates the peak corresponding to the desired FOXP3.

    Techniques Used: Expressing, Purification, Recombinant, Protein Purification, Affinity Chromatography, Chromatography, SDS Page, Molecular Weight, Marker, Staining

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Disease-linked regulatory DNA variants and homeostatic transcription factors in epidermis
    Article Snippet: DNA was extracted by a Zymo DNA Clean and Concentrator (Zymo Research, D4014), eluting in 27 μL water. .. Library was prepared using the NEB Ultra II DNA library prep kit (NEB, E7103S) following dx.doi.org/10.17504/protocols.io.bagaibse , with the following modifications: the adapter was diluted 20–25 fold, PCR was performed with SYBR dye to visualize amplification, and after PCR, 20 μL of water, then 30 μL of beads (0.6X instead of 0.8X) beads were added for the initial selection to remove large small fragments, followed by the second size selection using the addition of 20 μL beads (1X instead of 1.2X) for library capture. ..

    Article Title: Disease-linked regulatory DNA variants and homeostatic transcription factors in epidermis.
    Article Snippet: DNA was extracted by a Zymo DNA Clean and Concentrator (Zymo Research, D4014), eluting in 27μL water. .. Library was prepared using the NEB Ultra II DNA library prep kit (NEB, E7103S) following dx.doi.org/10.17504/protocols.io.bagaibse116, with the following modifications: the adapter was diluted 20–25 fold, PCR was performed with SYBR dye to visualize amplification, and after PCR, 20μL of water, then 30μL of beads (0.6X instead of 0.8X) beads were added for the initial selection to remove large small fragments, followed by the second size selection using the addition of 20μL beads (1X insteadof 1.2X) for library capture. ..

    Article Title: Disease-Linked Regulatory DNA Variants and Homeostatic Transcription Factors in Epidermis
    Article Snippet: DNA was extracted by a Zymo DNA Clean and Concentrator (Zymo Research, D4014), eluting in 27 μL water. .. Library was prepared using the NEB Ultra II DNA library prep kit (NEB, E7103S) following dx.doi.org/10.17504/protocols.io.bagaibse , with the following modifications: the adapter was diluted 20-25 fold, PCR was performed with SYBR dye to visualize amplification, and after PCR, 20 μL of water, then 30 μL of beads (0.6X instead of 0.8X) beads were added for the initial selection to remove large small fragments, followed by the second size selection using the addition of 20 μL beads (1X instead of 1.2X) for library capture. ..

    Amplification:

    Article Title: Disease-linked regulatory DNA variants and homeostatic transcription factors in epidermis
    Article Snippet: DNA was extracted by a Zymo DNA Clean and Concentrator (Zymo Research, D4014), eluting in 27 μL water. .. Library was prepared using the NEB Ultra II DNA library prep kit (NEB, E7103S) following dx.doi.org/10.17504/protocols.io.bagaibse , with the following modifications: the adapter was diluted 20–25 fold, PCR was performed with SYBR dye to visualize amplification, and after PCR, 20 μL of water, then 30 μL of beads (0.6X instead of 0.8X) beads were added for the initial selection to remove large small fragments, followed by the second size selection using the addition of 20 μL beads (1X instead of 1.2X) for library capture. ..

    Article Title: Disease-linked regulatory DNA variants and homeostatic transcription factors in epidermis.
    Article Snippet: DNA was extracted by a Zymo DNA Clean and Concentrator (Zymo Research, D4014), eluting in 27μL water. .. Library was prepared using the NEB Ultra II DNA library prep kit (NEB, E7103S) following dx.doi.org/10.17504/protocols.io.bagaibse116, with the following modifications: the adapter was diluted 20–25 fold, PCR was performed with SYBR dye to visualize amplification, and after PCR, 20μL of water, then 30μL of beads (0.6X instead of 0.8X) beads were added for the initial selection to remove large small fragments, followed by the second size selection using the addition of 20μL beads (1X insteadof 1.2X) for library capture. ..

    Article Title: Disease-Linked Regulatory DNA Variants and Homeostatic Transcription Factors in Epidermis
    Article Snippet: DNA was extracted by a Zymo DNA Clean and Concentrator (Zymo Research, D4014), eluting in 27 μL water. .. Library was prepared using the NEB Ultra II DNA library prep kit (NEB, E7103S) following dx.doi.org/10.17504/protocols.io.bagaibse , with the following modifications: the adapter was diluted 20-25 fold, PCR was performed with SYBR dye to visualize amplification, and after PCR, 20 μL of water, then 30 μL of beads (0.6X instead of 0.8X) beads were added for the initial selection to remove large small fragments, followed by the second size selection using the addition of 20 μL beads (1X instead of 1.2X) for library capture. ..

    Selection:

    Article Title: Disease-linked regulatory DNA variants and homeostatic transcription factors in epidermis
    Article Snippet: DNA was extracted by a Zymo DNA Clean and Concentrator (Zymo Research, D4014), eluting in 27 μL water. .. Library was prepared using the NEB Ultra II DNA library prep kit (NEB, E7103S) following dx.doi.org/10.17504/protocols.io.bagaibse , with the following modifications: the adapter was diluted 20–25 fold, PCR was performed with SYBR dye to visualize amplification, and after PCR, 20 μL of water, then 30 μL of beads (0.6X instead of 0.8X) beads were added for the initial selection to remove large small fragments, followed by the second size selection using the addition of 20 μL beads (1X instead of 1.2X) for library capture. ..

    Article Title: Disease-linked regulatory DNA variants and homeostatic transcription factors in epidermis.
    Article Snippet: DNA was extracted by a Zymo DNA Clean and Concentrator (Zymo Research, D4014), eluting in 27μL water. .. Library was prepared using the NEB Ultra II DNA library prep kit (NEB, E7103S) following dx.doi.org/10.17504/protocols.io.bagaibse116, with the following modifications: the adapter was diluted 20–25 fold, PCR was performed with SYBR dye to visualize amplification, and after PCR, 20μL of water, then 30μL of beads (0.6X instead of 0.8X) beads were added for the initial selection to remove large small fragments, followed by the second size selection using the addition of 20μL beads (1X insteadof 1.2X) for library capture. ..

    Article Title: Disease-Linked Regulatory DNA Variants and Homeostatic Transcription Factors in Epidermis
    Article Snippet: DNA was extracted by a Zymo DNA Clean and Concentrator (Zymo Research, D4014), eluting in 27 μL water. .. Library was prepared using the NEB Ultra II DNA library prep kit (NEB, E7103S) following dx.doi.org/10.17504/protocols.io.bagaibse , with the following modifications: the adapter was diluted 20-25 fold, PCR was performed with SYBR dye to visualize amplification, and after PCR, 20 μL of water, then 30 μL of beads (0.6X instead of 0.8X) beads were added for the initial selection to remove large small fragments, followed by the second size selection using the addition of 20 μL beads (1X instead of 1.2X) for library capture. ..

    Size Selection:

    Article Title: Disease-linked regulatory DNA variants and homeostatic transcription factors in epidermis
    Article Snippet: DNA was extracted by a Zymo DNA Clean and Concentrator (Zymo Research, D4014), eluting in 27 μL water. .. Library was prepared using the NEB Ultra II DNA library prep kit (NEB, E7103S) following dx.doi.org/10.17504/protocols.io.bagaibse , with the following modifications: the adapter was diluted 20–25 fold, PCR was performed with SYBR dye to visualize amplification, and after PCR, 20 μL of water, then 30 μL of beads (0.6X instead of 0.8X) beads were added for the initial selection to remove large small fragments, followed by the second size selection using the addition of 20 μL beads (1X instead of 1.2X) for library capture. ..

    Article Title: Disease-linked regulatory DNA variants and homeostatic transcription factors in epidermis.
    Article Snippet: DNA was extracted by a Zymo DNA Clean and Concentrator (Zymo Research, D4014), eluting in 27μL water. .. Library was prepared using the NEB Ultra II DNA library prep kit (NEB, E7103S) following dx.doi.org/10.17504/protocols.io.bagaibse116, with the following modifications: the adapter was diluted 20–25 fold, PCR was performed with SYBR dye to visualize amplification, and after PCR, 20μL of water, then 30μL of beads (0.6X instead of 0.8X) beads were added for the initial selection to remove large small fragments, followed by the second size selection using the addition of 20μL beads (1X insteadof 1.2X) for library capture. ..

    Article Title: Disease-Linked Regulatory DNA Variants and Homeostatic Transcription Factors in Epidermis
    Article Snippet: DNA was extracted by a Zymo DNA Clean and Concentrator (Zymo Research, D4014), eluting in 27 μL water. .. Library was prepared using the NEB Ultra II DNA library prep kit (NEB, E7103S) following dx.doi.org/10.17504/protocols.io.bagaibse , with the following modifications: the adapter was diluted 20-25 fold, PCR was performed with SYBR dye to visualize amplification, and after PCR, 20 μL of water, then 30 μL of beads (0.6X instead of 0.8X) beads were added for the initial selection to remove large small fragments, followed by the second size selection using the addition of 20 μL beads (1X instead of 1.2X) for library capture. ..

    Sequencing:

    Article Title: Distinct melanocyte subpopulations defined by stochastic expression of proliferation or maturation programs enable a rapid and sustainable pigmentation response
    Article Snippet: .. Libraries were prepared using NEB Ultra II DNA library prep kit (E7103S) following manufacturer’s instructions and sequencing was done, after pooling the libraries together, on the NextSeq 2000 platform. ..



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    Image Search Results


    Expression and purification of recombinant His-MBP-FOXP3(ΔN) protein (A) Schematic flowchart of the protein purification process, including Ni-NTA affinity chromatography followed by HiTrap Heparin HP chromatography. (B) SDS-PAGE analysis of purified fractions during the HiTrap Heparin HP chromatography. The arrow indicates the target protein. M represents the molecular weight marker, with numbers on the right indicating molecular weights in kDa. The gel was stained with Coomassie Blue dye. (C) The HiTrap Heparin HP affinity chromatography profile of the final purified protein. An asterisk indicates the peak corresponding to the desired FOXP3.

    Journal: STAR Protocols

    Article Title: Protocol for the genome-wide identification of intrinsic transcription factor binding motifs by mammalian-optimized pull-down sequencing

    doi: 10.1016/j.xpro.2026.104513

    Figure Lengend Snippet: Expression and purification of recombinant His-MBP-FOXP3(ΔN) protein (A) Schematic flowchart of the protein purification process, including Ni-NTA affinity chromatography followed by HiTrap Heparin HP chromatography. (B) SDS-PAGE analysis of purified fractions during the HiTrap Heparin HP chromatography. The arrow indicates the target protein. M represents the molecular weight marker, with numbers on the right indicating molecular weights in kDa. The gel was stained with Coomassie Blue dye. (C) The HiTrap Heparin HP affinity chromatography profile of the final purified protein. An asterisk indicates the peak corresponding to the desired FOXP3.

    Article Snippet: Alternatives: This protocol uses NEBNext Ultra II DNA Library Prep Kit with Sample Purification Beads (NEB #E7103S) to construct library.

    Techniques: Expressing, Purification, Recombinant, Protein Purification, Affinity Chromatography, Chromatography, SDS Page, Molecular Weight, Marker, Staining

    Library preparation Schematic diagram illustrating the major steps of library construction using the NEBNext Ultra II DNA Library Prep Kit. The workflow includes. (1) end repair and dA-tailing of fragmented genomic DNA. (2) adaptor ligation (hairpin adaptor containing dU); USER enzyme cleavage to open the adaptor, and (3) PCR enrichment with indexed primers to generate the final sequencing library.

    Journal: STAR Protocols

    Article Title: Protocol for the genome-wide identification of intrinsic transcription factor binding motifs by mammalian-optimized pull-down sequencing

    doi: 10.1016/j.xpro.2026.104513

    Figure Lengend Snippet: Library preparation Schematic diagram illustrating the major steps of library construction using the NEBNext Ultra II DNA Library Prep Kit. The workflow includes. (1) end repair and dA-tailing of fragmented genomic DNA. (2) adaptor ligation (hairpin adaptor containing dU); USER enzyme cleavage to open the adaptor, and (3) PCR enrichment with indexed primers to generate the final sequencing library.

    Article Snippet: NEBNext Ultra II DNA Library Prep with Sample Purification Beads , NEB , Cat#E7103S.

    Techniques: Ligation, Sequencing

    Library preparation Schematic diagram illustrating the major steps of library construction using the NEBNext Ultra II DNA Library Prep Kit. The workflow includes. (1) end repair and dA-tailing of fragmented genomic DNA. (2) adaptor ligation (hairpin adaptor containing dU); USER enzyme cleavage to open the adaptor, and (3) PCR enrichment with indexed primers to generate the final sequencing library.

    Journal: STAR Protocols

    Article Title: Protocol for the genome-wide identification of intrinsic transcription factor binding motifs by mammalian-optimized pull-down sequencing

    doi: 10.1016/j.xpro.2026.104513

    Figure Lengend Snippet: Library preparation Schematic diagram illustrating the major steps of library construction using the NEBNext Ultra II DNA Library Prep Kit. The workflow includes. (1) end repair and dA-tailing of fragmented genomic DNA. (2) adaptor ligation (hairpin adaptor containing dU); USER enzyme cleavage to open the adaptor, and (3) PCR enrichment with indexed primers to generate the final sequencing library.

    Article Snippet: Alternatives: This protocol uses NEBNext Ultra II DNA Library Prep Kit with Sample Purification Beads (NEB #E7103S) to construct library.

    Techniques: Ligation, Sequencing